Association of p60c-src with endosomal membranes in mammalian fibroblasts.

We have examined the subcellular localization of p60c-src in mammalian fibroblasts. Analysis of indirect immunofluorescence by three-dimensional optical sectioning microscopy revealed a granular cytoplasmic staining that co-localized with the microtubule organizing center. Immunofluorescence experiments with antibodies against a number of membrane markers demonstrated a striking co-localization between p60c-src and the cation-dependent mannose-6-phosphate receptor (CI-MPR), a marker that identifies endosomes. Both p60c-src and the CI-MPR were found to cluster at the spindle poles throughout mitosis. In addition, treatment of interphase and mitotic cells with brefeldin A resulted in a clustering of p60c-src and CI-MPR at a peri-centriolar position. Biochemical fractionation of cellular membranes showed that a major proportion of p60c-src co-enriched with endocytic membranes. Treatment of membranes containing HRP to alter their apparent density also altered the density of p60c-src-containing membranes. Similar density shift experiments with total cellular membranes revealed that the majority of membrane-associated p60c-src in the cell is associated with endosomes, while very little is associated with plasma membranes. These results support a role for p60c-src in the regulation of endosomal membranes and protein trafficking

Environmental stress alters genetic regulation of novelty seeking in vervet monkeys.

Considerable attention has been paid to identifying genetic influences and gene-environment interactions that increase vulnerability to environmental stressors, with promising but inconsistent results. A nonhuman primate model is presented here that allows assessment of genetic influences in response to a stressful life event for a behavioural trait with relevance for psychopathology. Genetic and environmental influences on free-choice novelty seeking behaviour were assessed in a pedigreed colony of vervet monkeys before and after relocation from a low stress to a higher stress environment. Heritability of novelty seeking scores, and genetic correlations within and between environments were conducted using variance components analysis. The results showed that novelty seeking was markedly inhibited in the higher stress environment, with effects persisting across a 2-year period for adults but not for juveniles. There were significant genetic contributions to novelty seeking scores in each year (h(2) = 0.35-0.43), with high genetic correlations within each environment (rhoG > 0.80) and a lower genetic correlation (rhoG = 0.35, non-significant) between environments. There were also significant genetic contributions to individual change scores from before to after the move (h(2) = 0.48). These results indicate that genetic regulation of novelty seeking was modified by the level of environmental stress, and they support a role for gene-environment interactions in a behavioural trait with relevance for mental health

A Report on the Media and the Immigration Debate

Analyzes media coverage of immigration since 1980 and how industry practices and new media have conditioned the public to associate immigration with illegality, crisis, controversy, and government failure, causing a stalemate in the policy debate

A major advance in powder metallurgy

Ultramet has developed a process which promises to significantly increase the mechanical properties of powder metallurgy (PM) parts. Current PM technology uses mixed powders of various constituents prior to compaction. The homogeneity and flaw distribution in PM parts depends on the uniformity of mixing and the maintenance of uniformity during compaction. Conventional PM fabrication processes typically result in non-uniform distribution of the matrix, flaw generation due to particle-particle contact when one of the constituents is a brittle material, and grain growth caused by high temperature, long duration compaction processes. Additionally, a significant amount of matrix material is usually necessary to fill voids and create 100 percent dense parts. In Ultramet's process, each individual particle is coated with the matrix material, and compaction is performed by solid state processing. In this program, Ultramet coated 12-micron tungsten particles with approximately 5 wt percent nickel/iron. After compaction, flexure strengths were measured 50 percent higher than those achieved in conventional liquid phase sintered parts (10 wt percent Ni/Fe). Further results and other material combinations are discussed

Editing contributed scholarly articles from a language management perspective

Taking language management as its initial perspective, this paper examines some of the sorts of linguistic problems that second language writers of English face when contributing to scholarly journals and some of the issues that editors face when working with authors on those problems. Language management theory (hereafter LMT) is briefly explained. Drawing on a substantial corpus (slightly less than 500,000 words), illustrations of various categories of problem types are provided. One finding shows that it is difficult, in practice, to differentiate between simple language management issues and organized language management issues, because what may appear to be simple management issues may in fact have extended implications. Some problem types are not unique to non-native speakers, but appear with different frequency and distribution in non-native speaker texts as compared with native-speaker texts. Some ethical questions implicit in editing non-native speaker texts are explored

Language Policy and Planning in Botswana, Malawi, Mozambique and South Africa: Some Common Issues

This volume covers the language situation in Botswana, Malawi, Mozambique and South Africa explaining the linguistic diversity, the historical and political contexts and the current language situation, including language-in-education planning, the role of the media, the role of religion, and the roles of non-indigenous languages. The authors are indigenous and have been participants in the language planning context. Botswana, Malawi and Mozambique are not well represented in the international language policy/planning literature, while the South African section draws together the published literature in this area. The purpose of the volumes in this series is to present up-to-date information on polities that are not well-known to researchers in the field. A longer range purpose is to collect comparable information on as many polities as possible in order to facilitate the development of a richer theory to guide language policy and planning in other polities that undertake the development of a national policy on languages. This volume is part of a areal series which is committed to providing descriptions of language planning and policy in countries around the world

Compounds and Methods for Reducing the Occurrence of Post-Surgical Adhesions

Compounds and methods for reducing the occurrence of a post-surgical adhesion are provided. The compounds can include acrylic acid groups and ethylene glycol groups, and can be directed to an area of damaged tissue by the incorporation of a fibrin targeting peptide. The compounds can further include a brush-like portion, capable of creating a steric barrier between a damaged tissue or organ and adjacent tissues or organs, and a targeting portion, capable of directing the compounds to a damaged tissue or organ. Methods of detecting damaged tissue and kits are also provided

Anomaly Cancellation in 2+1 dimensions in the presence of a domainwall mass

A Fermion in 2+1 dimensions, with a mass function which depends on one spatial coordinate and passes through a zero ( a domain wall mass), is considered. In this model, originally proposed by Callan and Harvey, the gauge variation of the effective gauge action mainly consists of two terms. One comes from the induced Chern-Simons term and the other from the chiral fermions, bound to the 1+1 dimensional wall, and they are expected to cancel each other. Though there exist arguments in favour of this, based on the possible forms of the effective action valid far from the wall and some facts about theories of chiral fermions in 1+1 dimensions, a complete calculation is lacking. In this paper we present an explicit calculation of this cancellation at one loop valid even close to the wall. We show that, integrating out the ``massive'' modes of the theory does produce the Chern-Simons term, as appreciated previously. In addition we show that it generates a term that softens the high energy behaviour of the 1+1 dimensional effective chiral theory thereby resolving an ambiguity present in a general 1+1 dimensional theory.Comment: 17 pages, LaTex file, CU-TP-61

Advancing the link between ocean connectivity, ecological function and management challenges

Ocean connectivity is a dynamic and rapidly evolving field of research in marine science, partly because there is an increasing demand for information on connectivity that informs effective assessment and management of marine resources. Achieving this will require a better alignment between ocean connectivity tools and developments and the needs and challenges of assessments and conservation. For these reasons, the ICES Journal of Marine Science solicited contributions to the article theme set (TS), Beyond ocean connectivity. We briefly summarize the nine articles that appear herein, grouping them into four general topics: methodological advances, population dynamics and assessment implications of connectivity, spatial and management implications, and connectivity in ecosystem processes. We also discuss the challenges facing ocean connectivity research if it is to effectively support advancing fisheries assessment frameworks and integrated ecosystem approaches. We hope that the contributions included in this TS serve to convince managers and fisheries scientists of the need to incorporate results from research on connectivity